Why N 10 HCL Is Used In Hb Estimation?

What are the 3 types of hemoglobin?

The other three forms of haemoglobin are present at much lower levels and are:Haemoglobin Gower II (α2ε2) – Composed of two alpha and two epsilon chains.Haemoglobin Portland I (ζ2γ2) – Comprised of two zeta and two gamma polypeptides.Haemoglobin Portland II (ζ2β2) – Made of two zeta and two beta polypeptide chains..

Which method is best for Hb estimation?

Direct cyanmethemoglobin method has been the gold standard for hemoglobin estimation but other methods like hemoglobin color scale, Sahli technique, Lovibond-Drabkin technique, Tallqvist technique, copper-sulfate method, HemoCue and automated haematology analyzers are also available.

Which anticoagulant should be used for Hb estimation?

EDTA anticoagulantMost of the commercial kits for HbA1c estimation requires sample to be collected in EDTA anticoagulant.

What is acid Hematin?

Black to brown amorphous to microcrystalline granules are encountered in histologic sections prepared from tissues fixed in formalin having a low pH. This pigment is produced by acid acting upon hemoglobin and is known as formalin pigment or acid hematin.

Which hemoglobin is detected by Sahli’s method?

Blood is mixed in a solution containing potassium cyanide and potassium ferricyanide. The potassium ferricyanide converts Hb to methemoglobin which is converted to cyanmethemoglobin (HiCN) by potassium cyanide. The absorbance of the solution is then measured in a spectrophotometer at a wavelength of 540nm.

What is a normal HB?

The normal range for hemoglobin is: For men, 13.5 to 17.5 grams per deciliter. For women, 12.0 to 15.5 grams per deciliter.

What is principle of acid hematin method of hemoglobin estimation?

Principle. When blood is mixed with an acid solution, the hemoglobin converts into the brown-colored acid hematin. The acid hematin is then diluted with distilled water till the color of the acid hematin matches that of the brown glass standard. The hemoglobin is estimated by reading the value directly from the scale.

What are the precautions to be taken during Haemoglobin estimation?

Wait for atleast 10 min for formation of Acid hematin.Tap water cannot be used as the pH varies and accurate values may not be obtained.Remove stirrer while adding distilled water and also while taking reading.More items…

Why is anticoagulant used?

Anticoagulants are medicines that help prevent blood clots. They’re given to people at a high risk of getting clots, to reduce their chances of developing serious conditions such as strokes and heart attacks. A blood clot is a seal created by the blood to stop bleeding from wounds.